Profiling of Humoral Immune Response in Typhoid Patients against Differentially Extracted Whole Cell Bacterial Protein Derived from S. typhi and S. spp

Typhoid fever is a multiorgan infectious disease caused by Salmonella typhi. It transmitted through fecal oral route and can be fatal without proper treatment. Therefore, early diagnosis of typhoid crucial. In the previous study, we have developed TYPHOIDYNE EIA, which showed excellent synergy between genus conserved species-specific antigens in serodiagnosis fever. EIA effectively detect differentiate patients, vaccinated subjects, healthy subjects with other febrile illnesses. Following successful development this report, further characterize antigenic components differentially extracted S. typhi spp recognized IgM, IgG, IgA antibody isotypes patients possible carrier western blot (WB) assay. The WB characterization revealed dynamic pattern recognition, significant variations number bands observed arrays antigens. reactivity patient’s sera was divided into 3 regions, region 1 (≥55 kDa) showing strongest followed 2 (54 kDa–34 (<34 kDa). Overall, good expressed these suggests potential role proteins differentiating carrier. highlighted study are also identified as prospective biomarkers for diagnostic use vaccine development.